成像角度與葉齡對葉片葉綠素熒光成像的影響研究
2023年電子技術應用第5期
楊振宇1,2,湯浩1,2,葛瑋1,2,夏倩1,2,仝德之3,付麗疆1,2,郭亞1,2
(1.江南大學 “輕工過程先進控制”教育部重點實驗室,江蘇 無錫 214122; 2.江南大學 物聯網工程學院,江蘇 無錫 214026;3.綠視芯科技(無錫)有限公司,江蘇 無錫 214000)
摘要: 葉綠素熒光成像作為一種高效的植物光合作用和生理狀態(tài)信息獲取手段,常被應用于智慧農業(yè)信息感知等眾多領域?,F階段的大尺度葉綠素熒光成像技術缺少與微觀的葉片級葉綠素熒光成像之間的機制上聯系,忽略成像角度與葉片葉齡對葉綠素熒光成像產生的影響,限制了其在大尺度農業(yè)智能評估與決策中應發(fā)揮的作用。針對上述問題,設計了一套可多角度成像的葉綠素熒光采集設備,用以探究葉綠素熒光成像與成像角度和葉片葉齡之間存在的聯系,并利用綠蘿、樟樹和大葉黃楊這三種分別代表藤本、喬木和灌木的植物共計60片葉子進行成像實驗,分析熒光強度、F_v/F_m和R_fd三個典型熒光指標。實驗結果表明成像角度對葉綠素熒光成像存在顯著影響,成像角度的增大會導致熒光參數值的減小,對不同種類植物的影響也不同。葉片的葉齡也會影響葉綠素熒光成像,成長期葉片的成像參數指標優(yōu)于成熟期葉片。
中圖分類號:S-3
文獻標志碼:A
DOI: 10.16157/j.issn.0258-7998.233870
中文引用格式: 楊振宇,湯浩,葛瑋,等. 成像角度與葉齡對葉片葉綠素熒光成像的影響研究[J]. 電子技術應用,2023,49(5):14-18.
英文引用格式: Yang Zhenyu,Tang Hao,Ge Wei,et al. Study on the effects of imaging angle and leaf age on leaf chlorophyll fluorescence imaging[J]. Application of Electronic Technique,2023,49(5):14-18.
文獻標志碼:A
DOI: 10.16157/j.issn.0258-7998.233870
中文引用格式: 楊振宇,湯浩,葛瑋,等. 成像角度與葉齡對葉片葉綠素熒光成像的影響研究[J]. 電子技術應用,2023,49(5):14-18.
英文引用格式: Yang Zhenyu,Tang Hao,Ge Wei,et al. Study on the effects of imaging angle and leaf age on leaf chlorophyll fluorescence imaging[J]. Application of Electronic Technique,2023,49(5):14-18.
Study on the effects of imaging angle and leaf age on leaf chlorophyll fluorescence imaging
Yang Zhenyu1,2,Tang Hao1,2,Ge Wei1,2,Xia Qian1,2,Tong Dezhi3,Fu Lijiang1,2,Guo Ya1,2
(1.Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Jiangnan University, Wuxi 214122, China; 2.School of IoT, Jiangnan University, Wuxi 214122, China; 3.Chloview Science and Technology (Wuxi) Co., Ltd., Wuxi 214000, China)
Abstract: Chlorophyll fluorescence imaging, as an efficient means of obtaining plant photosynthesis and physiological state information, is often used in many fields such as smart agricultural information perception. The current large-scale chlorophyll fluorescence imaging technology lacks the mechanistic connection with the microscopic leaf-level chlorophyll fluorescence imaging, ignoring the impact of imaging angle and leaf age on chlorophyll fluorescence imaging, which limits its application in large-scale agricultural intelligence assessment and role in decision-making. Aiming at the above problems, this paper designed a set of chlorophyll fluorescence acquisition equipment capable of multi-angle imaging to explore the relationship between chlorophyll fluorescence imaging, imaging angle and leaf age.A total of 60 leaves of epipremnum aureum,camphor and euonymus, representing vines, trees and shrubs were subjected to imaging experiments, and the fluorescence intensity, F_v/F_mand R_fd three typical fluorescence indicators were analyzed. The experimental results show that the imaging angle has a significant impact on the chlorophyll fluorescence imaging, and the increase of the imaging angle will lead to the decrease of the fluorescence parameter value, and the effect on different types of plants is also different. Leaf age also affects chlorophyll fluorescence imaging, and the imaging parameters of growing leaves are better than those of mature leaves.
Key words : photosynthesis;chlorophyll fluorescence imaging;imaging angle;leaf age
0 引言
農業(yè)作為基礎產業(yè),其產量關系糧食安全和經濟建設,農作物的產量與其生理狀態(tài)密不可分,農業(yè)信息化是實現智慧農業(yè)的必然條件,如何準確有效地檢測植物的生理狀態(tài)尤其是實現大尺度檢測一直是熱點研究話題。植物在進行光合作用的過程中,將吸收的太陽能轉化為自身所需的化學能、熱能以及葉綠素熒光。葉綠素熒光蘊含著豐富的植物生理生化信息,被稱為植物光合作用的探針,其分布和變化特征可反映植物的光合作用,進而可反映植物當前的生理狀態(tài)。葉綠素熒光可以通過手持式儀器針對具體葉面進行測量,也可以通過遙感的方法進行大尺度測量。因此,葉綠素熒光是目前植物生理學研究和植物遙感研究的重要手段之一,在智慧農業(yè)的信息獲取與評估決策中具有重要的價值。
本文詳細內容請下載:http://ihrv.cn/resource/share/2000005302
作者信息:
楊振宇1,2,湯浩1,2,葛瑋1,2,夏倩1,2,仝德之3,付麗疆1,2,郭亞1,2
(1.江南大學 “輕工過程先進控制”教育部重點實驗室,江蘇 無錫 214122;2.江南大學 物聯網工程學院,江蘇 無錫 214026;3.綠視芯科技(無錫)有限公司,江蘇 無錫 214000)
此內容為AET網站原創(chuàng),未經授權禁止轉載。